Difference between revisions of "Part:BBa K1611000"
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==MIT MAHE 2020== | ==MIT MAHE 2020== | ||
'''Summary''' | '''Summary''' | ||
| − | INF-gamma is a cytokine which has been shown to have important roles in tissue homeostasis, immune and inflammatory responses and immunosurveillance of tumor. Its signaling activates the Janus kinase (JAK) which activates transcription 1 (STAT1) pathway. This is expression of classical interferon- stimulated genes that key immune effector functions. Studies have shown effects of IFNγ on other leukocytes, vascular cells, adipose tissue cells, neurons and tumour cells that have important implications for autoimmunity, metabolic diseases, atherosclerosis, neurological diseases and immune checkpoint blockade cancer therapy. | + | |
| + | INF-gamma is a cytokine which has been shown to have important roles in tissue homeostasis, immune and inflammatory responses and immunosurveillance of tumor. Its signaling activates the Janus kinase (JAK) which activates transcription 1 (STAT1) pathway. This is expression of classical interferon- stimulated genes that key immune effector functions. Studies have shown effects of IFNγ on other leukocytes, vascular cells, adipose tissue cells, neurons and tumour cells that have important implications for autoimmunity, metabolic diseases, atherosclerosis, neurological diseases and immune checkpoint blockade cancer therapy. | ||
==References== | ==References== | ||
1. Ivashkiv L. B. (2018). IFNγ: signalling, epigenetics and roles in immunity, metabolism, disease and cancer immunotherapy. Nature reviews. Immunology, 18(9), 545–558. https://doi.org/10.1038/s41577-018-0029-z | 1. Ivashkiv L. B. (2018). IFNγ: signalling, epigenetics and roles in immunity, metabolism, disease and cancer immunotherapy. Nature reviews. Immunology, 18(9), 545–558. https://doi.org/10.1038/s41577-018-0029-z | ||
Revision as of 15:59, 20 October 2020
IFNgamma
Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory.
Part sequencing
Figure 1: Sequencing of IFN gamma cloned in pSB1C3 vector. The black line shows the consensus sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 33
MIT MAHE 2020
Summary
INF-gamma is a cytokine which has been shown to have important roles in tissue homeostasis, immune and inflammatory responses and immunosurveillance of tumor. Its signaling activates the Janus kinase (JAK) which activates transcription 1 (STAT1) pathway. This is expression of classical interferon- stimulated genes that key immune effector functions. Studies have shown effects of IFNγ on other leukocytes, vascular cells, adipose tissue cells, neurons and tumour cells that have important implications for autoimmunity, metabolic diseases, atherosclerosis, neurological diseases and immune checkpoint blockade cancer therapy.
References
1. Ivashkiv L. B. (2018). IFNγ: signalling, epigenetics and roles in immunity, metabolism, disease and cancer immunotherapy. Nature reviews. Immunology, 18(9), 545–558. https://doi.org/10.1038/s41577-018-0029-z