Difference between revisions of "Part:BBa K3582026"

Revision as of 15:45, 20 October 2020

Inhibitory sequence 1 of PfEMP1 - ICAM1 interaction grafted into a cyclotide

Inhibitory Sequence 1 for PfEMP-1 and ICAM-1 interaction

This biobrick is able to synthesize the grafted cyclotide that could inhibit the 5MZA interaction between the host protein (ICAM-1) and the parasite protein (PfEMP1). The different components of this biobrick are described below: 1] BBa_k1362401: C intein, which is the modified version of the biobrick used by the 2014 iGEM Heidelberg team. 2] BBa_k1362400: N intein, which is the modified version of the biobrick used by the 2014 iGEM Heidelberg team. 3] The cyclotide precursor: The gene containing the cyclotide Kalata B1(29 amino acids), which is inserted between the C intein and the N intein so that it will circularize after translation. 4] Strep Ⅱ tag: This tag is placed within loop 3 of the cyclotide construct which could reduce the hemolytic activity of the cyclotide. This tag is also used to purify the molecule. 5] BBa_kK3582006 : Inhibitory sequence 1, which is thought to be able to inhibit the interaction between ICAM-1 and PfEMP1, is grafted into loop 6 of the cyclotide. This inhibitor has a higher affinity for interaction as compared to the wild type.

This final product is thought to be able to act like a drug that is capable of preventing the ICAM-1 and PfEMP1 interaction.

Sequence and Features