Difference between revisions of "Part:BBa K3582004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | 1] RFC10 criteria satisfied | + | *1] RFC10 criteria satisfied |
| − | 2] 6x-His-tag gene added for purification | + | *2] 6x-His-tag gene added for purification |
| − | 3] Codon optimized with E-coli K-12 strain | + | *3] Codon optimized with E-coli K-12 strain |
| − | + | ||
| − | + | ||
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===Source=== | ===Source=== | ||
Revision as of 15:14, 20 October 2020
The DBLb domain of PF11_0521 PfEMP1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1407
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1113
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- 1] RFC10 criteria satisfied
- 2] 6x-His-tag gene added for purification
- 3] Codon optimized with E-coli K-12 strain
Source
Protein sequence was obtained from PDB and reverse translated to give this biobrick