Difference between revisions of "Part:BBa K3427001:Design"

 
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===Design Notes===
 
===Design Notes===
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BsaI sites were added to allow a Golden Gate
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BsaI sites were added to allow a Golden Gate after the entangled gene given by CAMEOS
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===Source===
 
===Source===

Latest revision as of 14:35, 20 October 2020


knt-gfp entangled CDSs for aminoglycoside resistance and GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 784
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 784
    Illegal BamHI site found at 378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 784
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 784
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BsaI sites were added to allow a Golden Gate after the entangled gene given by CAMEOS

Source

This part was designed with a software named Cameos (Thomas Blazejewski et al, Science ,09 Aug 2019:Vol. 365, Issue 6453, pp. 595-598, DOI: 10.1126/science.aav5477) to entangle this 2 coding genes : Knt and GFP

References