Difference between revisions of "Part:BBa K2622004"
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This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes. | This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes. | ||
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| + | ==References== | ||
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| + | Pleckaityte, M., Mistiniene, E., Lasickiene, R., Zvirblis, G., & Zvirbliene, A. (2011). Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis. BMC biotechnology, 11, 100. https://doi.org/10.1186/1472-6750-11-100 | ||
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Latest revision as of 14:29, 20 October 2020
scFv_antiVGY
Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.
This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.
References
Pleckaityte, M., Mistiniene, E., Lasickiene, R., Zvirblis, G., & Zvirbliene, A. (2011). Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis. BMC biotechnology, 11, 100. https://doi.org/10.1186/1472-6750-11-100
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41