Difference between revisions of "Part:BBa K3611008"
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When there are few viruses in the test sample, the fluorescent signal output by the engineered bacteria will be very weak. In order to ensure people’s health and safety as much as possible, we hope the detection limit of our detection system is low enough and the output level is high enough to ensure that trace viruses would not be missed. In order to achieve this, we designed a cascade amplifier to amplify the detected virus signal, to increase the expression of the reporter gene and lower the detection limit. | When there are few viruses in the test sample, the fluorescent signal output by the engineered bacteria will be very weak. In order to ensure people’s health and safety as much as possible, we hope the detection limit of our detection system is low enough and the output level is high enough to ensure that trace viruses would not be missed. In order to achieve this, we designed a cascade amplifier to amplify the detected virus signal, to increase the expression of the reporter gene and lower the detection limit. | ||
| + | The natural hrp regulatory network contains activator protein HrpR and HrpS. These two would form an ultrasensitive high-order co-complex HrpRS, which bind the upstream activator sequence of the hrpL promoter to remodel the closed σ54-RNAP-hrpL transcription complex to an open one through ATP hrdrolysis. This have been proven to amplify the input transcriptional signal. | ||
Revision as of 13:35, 20 October 2020
hrp Bio-Amplifier
When there are few viruses in the test sample, the fluorescent signal output by the engineered bacteria will be very weak. In order to ensure people’s health and safety as much as possible, we hope the detection limit of our detection system is low enough and the output level is high enough to ensure that trace viruses would not be missed. In order to achieve this, we designed a cascade amplifier to amplify the detected virus signal, to increase the expression of the reporter gene and lower the detection limit.
The natural hrp regulatory network contains activator protein HrpR and HrpS. These two would form an ultrasensitive high-order co-complex HrpRS, which bind the upstream activator sequence of the hrpL promoter to remodel the closed σ54-RNAP-hrpL transcription complex to an open one through ATP hrdrolysis. This have been proven to amplify the input transcriptional signal.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1897
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1879
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1089
Illegal SapI.rc site found at 1722