Difference between revisions of "Part:BBa K136046:Design"

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===References===
 
===References===
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EnvZ-OmpR Interaction and Osmoregulation in Escherichia coli, Sheng Jian Cai and Masayori Inouye (2002), THE JOURNAL OF BIOLOGICAL CHEMISTRY
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EnvZ-OmpR Interaction and Osmoregulation in Escherichia coli,  
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<br> Sheng Jian Cai and Masayori Inouye (2002),  
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<br> THE JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 277, No. 27

Revision as of 22:43, 28 October 2008

envZ*


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR and ligation in pCR4Blunt-TOPO


Source

bacterial genome of mutated (I281S) strain for EnvZ.

References

EnvZ-OmpR Interaction and Osmoregulation in Escherichia coli,
Sheng Jian Cai and Masayori Inouye (2002),
THE JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 277, No. 27