Difference between revisions of "Part:BBa K3402028"
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<partinfo>BBa_K3402028 short</partinfo>
 
<partinfo>BBa_K3402028 short</partinfo>
  
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This part consists of three short genes. <i>RS</i> and <i>HH</i>. are linked at the 3’ end of sg<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]), <i>HDV</i> is linked at the 5’ end of sg<i>GFP</i>.
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<i>HDV</i> is linked at the 5' end of sg<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]).
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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In the device that we want to express Cas9 protein, these three parts of genes will help in the formation of mature sgRNA.
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In the device that we want to express Cas9 gene, this gene will help in the formation of mature sgRNA.
  
 
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Revision as of 12:16, 20 October 2020


HDV

HDV is linked at the 5' end of sgGFP(BBa_K3402024).

Usage and Biology

In the device that we want to express Cas9 gene, this gene will help in the formation of mature sgRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 33
    Illegal NgoMIV site found at 62
  • 1000
    COMPATIBLE WITH RFC[1000]