Difference between revisions of "Part:BBa K3482000"

Revision as of 11:24, 20 October 2020

heat-inducible RNA-thermosensor 2U

The 2U RNA thermosensor is inserted directly upstream of the gene of interest. The gene expression is then modulated by temperature.

Usage and Biology

RNA thermosensors are non-coding RNA molecules that are thermosensitive. When place upstream of a gene, they can regulate the translation of this gene. RNA thermosensors usually act by changing in the secondary structure due to the change of temperature. These changes will block or free the acces to the ribosome binding site (RBS) to the ribosome and thus regulate the translation. Thermosensors are often present in heat-shock and cold-shock proteins regulation.

We used this RNA thermosensors to regulate the expression of a toxin or an antitoxin in our kill-switch

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482017 .

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 14
Illegal PstI site found at 20
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 14
Illegal PstI site found at 20
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 14
Illegal PstI site found at 20
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 14
Illegal PstI site found at 20
1000
COMPATIBLE WITH RFC[1000]

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 ===Characterization===
+All the data concerning the characterization of that part can be found in the composite part : <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3482004"> BBa_K3482017 </a></html>.
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