Difference between revisions of "Part:BBa K3425001"
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<partinfo>BBa_K3425001 short</partinfo> | <partinfo>BBa_K3425001 short</partinfo> | ||
− | + | ||
+ | ===iGEM Type IIS Vectors=== | ||
+ | ====pEven Loop Vectors (for Level 2)==== | ||
+ | {{CSS/DNA_Table}} | ||
+ | |||
+ | When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with <partinfo>BBa_J04455</partinfo>), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites. | ||
+ | |||
+ | <html> | ||
+ | <center> | ||
+ | <table class="dnaTable"> | ||
+ | <tr> | ||
+ | <th>Part number</th> | ||
+ | <th>Name</th> | ||
+ | <th>Loop Name</th> | ||
+ | <th>Fusion Site 5'</th> | ||
+ | <th>MTU</th> | ||
+ | <th>Fusion Site 3'</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></html>[[Part:BBa_K3425001|<partinfo>BBa_K3425001</partinfo>]]<html></td> | ||
+ | <td>pSB3C11</td> | ||
+ | <td>pEven1</td> | ||
+ | <td class="dnaSeq rightAlign">GGAG</td> | ||
+ | <td class="centerAlign partType" style="background-color: #00B050;">MTU 1</td> | ||
+ | <td class="dnaSeq">TACT</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></html>[[Part:BBa_K3425002|<partinfo>BBa_K3425002</partinfo>]]<html></td> | ||
+ | <td>pSB3C12</td> | ||
+ | <td>pEven2</td> | ||
+ | <td class="dnaSeq rightAlign">TACT</td> | ||
+ | <td class="centerAlign partType" style="background-color: #FFC001;">MTU 2</td> | ||
+ | <td class="dnaSeq">AATG</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></html>[[Part:BBa_K3425003|<partinfo>BBa_K3425003</partinfo>]]<html></td> | ||
+ | <td>pSB3C13</td> | ||
+ | <td>pEven3</td> | ||
+ | <td class="dnaSeq rightAlign">AATG</td> | ||
+ | <td class="centerAlign partType" style="background-color: #4472C4;">MTU 3</td> | ||
+ | <td class="dnaSeq">GCTT</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></html>[[Part:BBa_K3425004|<partinfo>BBa_K3425004</partinfo>]]<html></td> | ||
+ | <td>pSB3C14</td> | ||
+ | <td>pEven4</td> | ||
+ | <td class="dnaSeq rightAlign"> GCTT </td> | ||
+ | <td class="centerAlign partType" style="background-color: #FF0000;">MTU 4</td> | ||
+ | <td class="dnaSeq">CGCT</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </center> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | {{TypeIIS/References}} | ||
+ | |||
+ | '''Note:''' This section was adapted from [[Part:pSB3C01|<partinfo>pSB3C01</partinfo>]] in order to collect all the information in one page. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 10:50, 20 October 2020
pSB3C11: Improved pEven1 Loop Vector based on pSB3C01
iGEM Type IIS Vectors
pEven Loop Vectors (for Level 2)
When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with BBa_J04455), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites.
Part number | Name | Loop Name | Fusion Site 5' | MTU | Fusion Site 3' |
---|---|---|---|---|---|
BBa_K3425001 | pSB3C11 | pEven1 | GGAG | MTU 1 | TACT |
BBa_K3425002 | pSB3C12 | pEven2 | TACT | MTU 2 | AATG |
BBa_K3425003 | pSB3C13 | pEven3 | AATG | MTU 3 | GCTT |
BBa_K3425004 | pSB3C14 | pEven4 | GCTT | MTU 4 | CGCT |
The iGEM Type IIS assembly standard is based on MoClo and Loop
- A Modular Cloning System for Standardized Assembly of Multigene Constructs
- Loop assembly: a simple and open system for recursive fabrication of DNA circuits
Note: This section was adapted from pSB3C01 in order to collect all the information in one page.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal PstI site found at 12 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal NheI site found at 1399
Illegal PstI site found at 12 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal PstI site found at 12 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal PstI site found at 12
Illegal AgeI site found at 990
Illegal AgeI site found at 1313 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2719
Illegal BsaI.rc site found at 6