Difference between revisions of "Part:BBa K3487010"
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===Description===
 
===Description===
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This part can be used directly as a suicide switch to prevent engineered bacteria from escaping into the environment, and is suitable for a variety of common engineered bacteria, such as BL21 or DH5α.
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RS is a riboswitch regulated by lysine, which regulates the concentration of lysine to turn on or off downstream gene expression;RalR is a toxic protein that can cleave methylated and unmethylated DNA, leading to cell death.
  
 
<!-- Add more about the biology of this part here
 
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K3487010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3487010 SequenceAndFeatures</partinfo>
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===Result===
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We have constructed a suicide mechanism to ensure that the transgenic bacteria will not be harmful to the environment. The results of the construction of the recombinant plasmid pET28a-RS-RalR are shown in the following.
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===Reference===
 
===Reference===

Revision as of 09:22, 20 October 2020


A kill switch regulated by lysine concentration

Description

RS is a riboswitch regulated by lysine, which regulates the concentration of lysine to turn on or off downstream gene expression;RalR is a toxic protein that can cleave methylated and unmethylated DNA, leading to cell death.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 256

Result

We have constructed a suicide mechanism to ensure that the transgenic bacteria will not be harmful to the environment. The results of the construction of the recombinant plasmid pET28a-RS-RalR are shown in the following.


Reference

Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.
Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.