Difference between revisions of "Part:BBa K3402028"
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<partinfo>BBa_K3402028 short</partinfo> | <partinfo>BBa_K3402028 short</partinfo> | ||
| − | This part consists of three short genes. RS and HH are linked at the 3’ end of | + | This part consists of three short genes. <i>RS</i> and <i>HH</i>. are linked at the 3’ end of sg<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]), <i>HDV</i> is linked at the 5’ end of sg<i>GFP</i>. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:19, 20 October 2020
HDV
This part consists of three short genes. RS and HH. are linked at the 3’ end of sgGFP(BBa_K3402024), HDV is linked at the 5’ end of sgGFP.
Usage and Biology
In the device that we want to express Cas9 protein, these three parts of genes will help in the formation of mature sgRNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 33
Illegal NgoMIV site found at 62 - 1000COMPATIBLE WITH RFC[1000]