Difference between revisions of "Part:BBa K3351001"
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<partinfo>BBa_K3351001 short</partinfo> | <partinfo>BBa_K3351001 short</partinfo> | ||
| − | + | ===Summary=== | |
The ribosome binding site (RBS), defined as the region within the mRNA that is protected by the bound 30S subunit in the initiation complex, was reported to extend from positions –18 to +10 relative to the start codon. This RBS in plasmid PET28a is suitable for general protein expression in E. coli. Stable structures within an RBS should impair binding and interfere with initiation. Its local structure rather than the stability of an SD-antiSD interaction predicts output. So the accessibility of an RBS affects protein output. | The ribosome binding site (RBS), defined as the region within the mRNA that is protected by the bound 30S subunit in the initiation complex, was reported to extend from positions –18 to +10 relative to the start codon. This RBS in plasmid PET28a is suitable for general protein expression in E. coli. Stable structures within an RBS should impair binding and interfere with initiation. Its local structure rather than the stability of an SD-antiSD interaction predicts output. So the accessibility of an RBS affects protein output. | ||
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| + | ===Reference=== | ||
| + | [1]Sterk M, Romilly C, Wagner EGH. Unstructured 5'-tails act through ribosome standby to override inhibitory structure at ribosome binding sites. Nucleic Acids Res. 2018 May 4;46(8):4188-4199. doi: 10.1093/nar/gky073. PMID: 29420821; PMCID: PMC5934652. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:55, 19 October 2020
A ribosome binding site from pet28a plasmid.
Summary
The ribosome binding site (RBS), defined as the region within the mRNA that is protected by the bound 30S subunit in the initiation complex, was reported to extend from positions –18 to +10 relative to the start codon. This RBS in plasmid PET28a is suitable for general protein expression in E. coli. Stable structures within an RBS should impair binding and interfere with initiation. Its local structure rather than the stability of an SD-antiSD interaction predicts output. So the accessibility of an RBS affects protein output.
Reference
[1]Sterk M, Romilly C, Wagner EGH. Unstructured 5'-tails act through ribosome standby to override inhibitory structure at ribosome binding sites. Nucleic Acids Res. 2018 May 4;46(8):4188-4199. doi: 10.1093/nar/gky073. PMID: 29420821; PMCID: PMC5934652. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]