Difference between revisions of "Part:BBa K3423005:Design"

 
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===Design Notes===
 
===Design Notes===
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When anti-CRISPR (acr) promoter inhibitor Anti-CRISPR-associated 1 (Aca1) is mutated to Aca1R44A, Aca1R44A can no longer bind to the promoter region, therefore the acr promoter will not be inhibited. When green fluorescent protein is inserted after the acr promoter, the efficiency of the promoter can be measured.
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Through the experiments we did, we found Navitoclax can inhibit Aca1 effectively and thus increase genes downstream Acr promoter significantly.
  
 
===Source===
 
===Source===

Latest revision as of 13:38, 19 October 2020


a controlled protein expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1057


Design Notes

When anti-CRISPR (acr) promoter inhibitor Anti-CRISPR-associated 1 (Aca1) is mutated to Aca1R44A, Aca1R44A can no longer bind to the promoter region, therefore the acr promoter will not be inhibited. When green fluorescent protein is inserted after the acr promoter, the efficiency of the promoter can be measured.

Through the experiments we did, we found Navitoclax can inhibit Aca1 effectively and thus increase genes downstream Acr promoter significantly.

Source

Pseudomonas phages

References