Difference between revisions of "Part:BBa K3351009"
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| − | + | ===Summary=== | |
His tags have gained great popularity over the last decade as a purification tool for recombinant proteins. Cloning vectors generally introduce six consecutive histidines and an optional protease-cleavage site or linker to the N- or C-terminus of the protein of interest. These His tags facilitate selective binding of the expressed protein to a nickel-affinity column. The tag may then optionally be removed by a protease, requiring another purification step. | His tags have gained great popularity over the last decade as a purification tool for recombinant proteins. Cloning vectors generally introduce six consecutive histidines and an optional protease-cleavage site or linker to the N- or C-terminus of the protein of interest. These His tags facilitate selective binding of the expressed protein to a nickel-affinity column. The tag may then optionally be removed by a protease, requiring another purification step. | ||
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| + | ===Reference=== | ||
| + | [1] Smith, M. C., Furman, T. C., Ingolia, T. D. & Pidgeon, C. (1988). J. Biol. Chem. 263, 7211–7215 | ||
| + | [2] Carson M , Johnson D H , Mcdonald H , et al. His-tag impact on structure[J]. Acta Crystallographica, 2007, 63(3):295-301. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:31, 19 October 2020
His tag.
Summary
His tags have gained great popularity over the last decade as a purification tool for recombinant proteins. Cloning vectors generally introduce six consecutive histidines and an optional protease-cleavage site or linker to the N- or C-terminus of the protein of interest. These His tags facilitate selective binding of the expressed protein to a nickel-affinity column. The tag may then optionally be removed by a protease, requiring another purification step.
Reference
[1] Smith, M. C., Furman, T. C., Ingolia, T. D. & Pidgeon, C. (1988). J. Biol. Chem. 263, 7211–7215 [2] Carson M , Johnson D H , Mcdonald H , et al. His-tag impact on structure[J]. Acta Crystallographica, 2007, 63(3):295-301. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]