Difference between revisions of "Part:BBa K3506007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This part can be used to couple the transcription of the target gene with yeast cell cycle.CLB2 promoter is a promoter which promotes transciption in late S and G2/M of the yeast cell cycle .If you want to express target gene during cell division,you can place the Clb2 promoter sequence before your gene sequence on the yeast plasmid. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 13:21, 19 October 2020
CLB2 promoter
This part is the CLB2 promoter which is -829 to -365 nt relative to CLB2 ATG on the genome of Saccharomyces cerevisiae BY4741. CLB2 promoter is the promoter of the G2/mitotic-specific cyclin-2(CLB2) which accumulates steadily during G2 and is abruptly destroyed at mitosis. CLB2 interacts with the CDC2 protein kinase to form maturation-promoting factor(MPF) and is essential for the control of the cell cycle at the G2/M (mitosis) transition.Previous studies have revealed that CLB2 promoter can regulate the transcription of its downstream gene periodically. Under the control of CLB2 promoter, the downstream gene starts to transcript in the S phase, reaches a peak at the G2/M transition, and rapidly degrades at the end of the M phase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 229
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 203
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]