Difference between revisions of "Part:BBa K3402000"
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[[Image:Expression of yeGFP.png|700px|thumb|center|Expression of <i>yeGFP</i>]]
 
[[Image:Expression of yeGFP.png|700px|thumb|center|Expression of <i>yeGFP</i>]]
  
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===Contribution===
 
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Because <i>Starmerella bombicola</i> is eukaryote, origin GFP gene can not express in it. So we optimized the codon sequence of <i>yeGFP</i> at the basis of [https://parts.igem.org/Part:BBa_E0040# BBa_E0040]to express it in <i>Starmerella bombicola</i> to characterize the strength of promoters.
 
  
 
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Revision as of 12:09, 19 October 2020


yeGFP

The yeGFP can express green fluorescence protein, which can be used as a report gene to characterize the strength of promoters.

Usage and Biology

We link different promoters to yeGFP to express green fluorescent protein, so we will know the strength of promoters by testing different fluorescence intensity of different promoters

Expression of yeGFP


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13