Difference between revisions of "Part:BBa K3582005:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
1]Codon optimized with E-coli K-12 strain.
+
*1]Codon optimized with E-coli K-12 strain.
−
2]Inhibitor 1 grafted in loop 6
+
*2]Inhibitor 1 grafted in loop 6
−
3]C and N inteins added for circularization
+
*3]C and N inteins added for circularization
  
  

Latest revision as of 10:49, 19 October 2020


Inhibitory Sequence 1 Grafted In Cyclotide Kalata B1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 112
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 143
    Illegal BsaI.rc site found at 109


Design Notes

  • 1]Codon optimized with E-coli K-12 strain.
  • 2]Inhibitor 1 grafted in loop 6
  • 3]C and N inteins added for circularization



Source

Reverse translation of amino acid sequence

References