Difference between revisions of "Part:BBa K3409012"

Revision as of 07:29, 19 October 2020

Microcin PDI cluster gene for McpB

Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007) and a double terminator (BBa_B0015). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3409012 short</partinfo>
-Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.
+Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007) and a double terminator (BBa_B0015). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.
 <!-- Add more about the biology of this part here