Difference between revisions of "Part:BBa K3629007:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Codon optimized for expression and function in <i>Yarrowia lipolytica.</i>
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Codon optimized for expression and function in <i>Yarrowia lipolytica.</I> The native signal peptide from <I>T. reesei</i> was also removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i>
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===Source===
 
===Source===

Latest revision as of 02:47, 19 October 2020


Trichoderma reesei CBHII with FLAG tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 341
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression and function in Yarrowia lipolytica. The native signal peptide from T. reesei was also removed so it would not interfere with fused secretion tags native to Y. lipolytica

Source

From genomic sequence of Trichoderma reesei.

References