Difference between revisions of "Part:BBa K3629009"
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===Design=== | ===Design=== | ||
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| + | The native signal peptide from <I>T. reesei</i> was removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i> | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 02:46, 19 October 2020
Trichoderma reesei EGII with FLAG tag
Endoglucanase II coding sequence from Trichoderma reesei with FLAG tag.
Usage and Biology
Design
The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 283
Illegal NgoMIV site found at 817
Illegal NgoMIV site found at 1135 - 1000COMPATIBLE WITH RFC[1000]