Difference between revisions of "Part:BBa K3629006"
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===Design=== | ===Design=== | ||
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+ | The native signal peptide from <I>N. crassa</i> was removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i> | ||
===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 02:43, 19 October 2020
Neurospora crassa CBHI with Myc Tag
Cellobiohydrolase I coding sequence from Neurospora crassa with 6x Myc tag.
Usage and Biology
Design
The native signal peptide from N. crassa was removed so it would not interfere with fused secretion tags native to Y. lipolytica
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 585
Illegal BglII site found at 945 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1453
- 1000COMPATIBLE WITH RFC[1000]
References
Guo Z-P, Duquesne S, Bozonnet S, Cioci G, Nicaud J-M, Marty A, O’Donohue MJ. Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach. Biotechnology for Biofuels. 2017;10(1). doi:10.1186/s13068-017-0819-8