Difference between revisions of "Part:BBa K3629019"
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2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate leucine synthesis and produce a leucine overproducing strain. | 2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate leucine synthesis and produce a leucine overproducing strain. | ||
− | See BBa_K3629023 for a functional high expression construct using this coding sequence. | + | See [https://parts.igem.org/Part:BBa_K3629023 BBa_K3629023] for a functional high expression construct using this coding sequence. |
===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 02:40, 19 October 2020
Yarrowia lipolytica LEU4 gene
2-isopropylmalate (LEU4) coding sequence from Yarrowia lipolytica .
Usage and Biology
2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate leucine synthesis and produce a leucine overproducing strain.
See BBa_K3629023 for a functional high expression construct using this coding sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 728
Illegal BamHI site found at 293
Illegal XhoI site found at 428 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 799
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 544