Difference between revisions of "Part:BBa K3629020"
(→Usage and Biology) |
(→Usage and Biology) |
||
| Line 9: | Line 9: | ||
Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a tryptophan binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain. | Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a tryptophan binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain. | ||
| − | See BBa_K3629024 for a high expression construct using this coding sequence. | + | See [https://parts.igem.org/Part:BBa_K3629024 BBa_K3629024] for a high expression construct using this coding sequence. |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 02:40, 19 October 2020
Yarrowia lipolytica TRP2 Gene
Anthranilate synthase component 1 (TRP2) coding sequence Yarrowia lipolytica .
Usage and Biology
Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a tryptophan binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain.
See BBa_K3629024 for a high expression construct using this coding sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 943
Illegal NgoMIV site found at 1213
Illegal NgoMIV site found at 1294
Illegal NgoMIV site found at 1393 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 283
Illegal BsaI.rc site found at 544
Illegal BsaI.rc site found at 1441