Difference between revisions of "Part:BBa K3453162"

 
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<partinfo>BBa_K3453162 short</partinfo>
 
<partinfo>BBa_K3453162 short</partinfo>
  
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This part is a transcription unit of the Rosewood DmTrnL-UAA Trigger 1.2 ([[Part:BBa_K3453062|BBa_K3453062]]) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) and the strong SBa_000587 synthetic terminator ([[Part:BBa_K3453000|BBa_K3453000]]).
  
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===Usage and Biology===
 
===Usage and Biology===
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A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).
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[[File: T--Evry_Paris-Saclay--toehold-switch.png|600px]]
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Figure 1. Toehold switches principle.
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This part is a transcription unit of [[Part:BBa_K3453062|BBa_K3453062]] which is a trigger for the Rosewood DmTrnL-UAA Toehold Switch 1.2 ([[Part:BBa_K3453052|BBa_K3453052]]) and also contains the switch binding site for the Rosewood DmTrnL-UAA Toehold 2.3 ([[Part:BBa_K3453056|BBa_K3453056]]).
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This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.
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Full results are available on the [[Part:BBa_K3453152|BBa_K3453152]] and [[Part:BBa_K3453156|BBa_K3453156]] pages in the registry.
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===References===
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[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
  
 
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Revision as of 18:33, 18 October 2020


Rosewood DmTrnL-UAA Trigger 1.2 transcription unit

This part is a transcription unit of the Rosewood DmTrnL-UAA Trigger 1.2 (BBa_K3453062) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).

T--Evry Paris-Saclay--toehold-switch.png

Figure 1. Toehold switches principle.

This part is a transcription unit of BBa_K3453062 which is a trigger for the Rosewood DmTrnL-UAA Toehold Switch 1.2 (BBa_K3453052) and also contains the switch binding site for the Rosewood DmTrnL-UAA Toehold 2.3 (BBa_K3453056).

This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.

Full results are available on the BBa_K3453152 and BBa_K3453156 pages in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]