Difference between revisions of "Part:BBa K3487012"

 
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<partinfo>BBa_K3487012 short</partinfo>
 
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When the leader peptide pelB is connected to ComD, its role is to guide the histidine kinase receptor ComD to the membrane to form a membrane receptor protein, increase the binding rate of CSP and ComD, and enable it to better bind to downstream promoters.
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===Description===
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comD, as the sensory regulatory membrane protein in the two-component regulatory system of Streptococcus mutans, is very important in the function of transmitting signals. So in order to adapt comD to E. coli, we added a leader peptide before comD to help the expressed membrane protein comD attach to the cell membrane.
  
 
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<partinfo>BBa_K3487012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3487012 SequenceAndFeatures</partinfo>
  
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===Reference===
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【1】Extracellular expression of Thermobifida fusca cutinase with pelB signal peptide depends on more than type II secretion pathway in Escherichia coli[J]. Journal of Biotechnology, 2015, 204:47-52.
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【2】陈冰, 王金华, 陈娇,等. ComD基因缺陷对变异链球菌生物膜形成的影响[J]. 中国微生态学杂志, 2015, 27(009):1034-1037.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3487012 parameters</partinfo>
 
<partinfo>BBa_K3487012 parameters</partinfo>
 
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Revision as of 13:25, 18 October 2020


Histidine kinase receptor with leader peptide

Description

comD, as the sensory regulatory membrane protein in the two-component regulatory system of Streptococcus mutans, is very important in the function of transmitting signals. So in order to adapt comD to E. coli, we added a leader peptide before comD to help the expressed membrane protein comD attach to the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 120
    Illegal AgeI site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

【1】Extracellular expression of Thermobifida fusca cutinase with pelB signal peptide depends on more than type II secretion pathway in Escherichia coli[J]. Journal of Biotechnology, 2015, 204:47-52. 【2】陈冰, 王金华, 陈娇,等. ComD基因缺陷对变异链球菌生物膜形成的影响[J]. 中国微生态学杂志, 2015, 27(009):1034-1037.