Difference between revisions of "Part:BBa K3487007"
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===Description=== | ===Description=== | ||
Usually the suicide switch that prevents organisms from escaping will be set to auxotrophic type, which requires gene resection technology, and whether it is accurate is a big problem that needs to be faced. The riboswitch does not have these problems. The gene sequence is directly introduced into the required vector, and the switch directly forms a multiple stem-loop structure on the mRNA to stop translation. | Usually the suicide switch that prevents organisms from escaping will be set to auxotrophic type, which requires gene resection technology, and whether it is accurate is a big problem that needs to be faced. The riboswitch does not have these problems. The gene sequence is directly introduced into the required vector, and the switch directly forms a multiple stem-loop structure on the mRNA to stop translation. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:59, 18 October 2020
Lysine riboswitch
Description
Usually the suicide switch that prevents organisms from escaping will be set to auxotrophic type, which requires gene resection technology, and whether it is accurate is a big problem that needs to be faced. The riboswitch does not have these problems. The gene sequence is directly introduced into the required vector, and the switch directly forms a multiple stem-loop structure on the mRNA to stop translation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 12
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 284
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 256
References
【2】Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.