Difference between revisions of "Part:BBa K3431044"

Revision as of 11:26, 18 October 2020

zr146_B_ToeholdSwitch-Regulated Invertase

Toehold Switch with Invertase as Expression Protein (zr146_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-146 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-146 (zr146_B) (BBa_K3431035) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-146. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 40
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 40
Illegal NheI site found at 1421
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 40
Illegal BglII site found at 1192
Illegal BamHI site found at 1322
Illegal XhoI site found at 1393
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 40
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 40
Illegal AgeI site found at 993
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3431044 short</partinfo>
-This is an expression device with toehold switch regulatory mechanism.
+Toehold Switch with Invertase as Expression Protein (zr146_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-146 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-146 (zr146_B) (BBa_K3431035) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-146. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.
 <!-- Add more about the biology of this part here