Difference between revisions of "Part:BBa K3431043"

 
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<partinfo>BBa_K3431043 short</partinfo>
 
<partinfo>BBa_K3431043 short</partinfo>
  
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This is an expression device with toehold switch regulatory mechanism.
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Toehold Switch with Invertase as Expression Protein (oz21_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-21 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-21 (oz21_B) (BBa_K3431034) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-21. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.
  
 
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Revision as of 11:22, 18 October 2020


oz21_B_ToeholdSwitch-Regulated Invertase

Toehold Switch with Invertase as Expression Protein (oz21_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-21 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-21 (oz21_B) (BBa_K3431034) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-21. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1201
    Illegal BamHI site found at 1331
    Illegal XhoI site found at 1402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1002
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 533