Difference between revisions of "Part:BBa K3524005:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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We made a synonymous mutation, mutating the 241st. C to T in Part: BBa_K3524003 to remove the BsaI restriction site to meet the type IIS standard.
 
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===Source===
 
===Source===

Latest revision as of 09:18, 18 October 2020


NahR-Psal-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The bio-sensor (nahR and Psal) comes from the genomic sequence of Pseudomonas Stutzeri.

References