Difference between revisions of "Part:BBa K3431016"

Revision as of 08:57, 18 October 2020

pp21_B_ToeholdSwitch-Regulated Invertase

Toehold Switch with Invertase as Expression Protein (pp21_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-21 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-21 (pp21_B) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-21.

Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1419
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1190
Illegal BamHI site found at 1320
Illegal XhoI site found at 1391
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 991
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3431016 short</partinfo>
-Toehold Switch with Invertase as Expression Protein (pp21_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-21 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) ([BBa_K3431000](https://parts.igem.org/Part:BBa_K3431000)), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-21 (pp21_B) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-21.
+Toehold Switch with Invertase as Expression Protein (pp21_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-21 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-21 (pp21_B) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-21.
-Furthermore, we include T7 promoter  ([BBa_I719005](https://parts.igem.org/Part:BBa_I719005)) and terminator ([BBa_K731721](https://parts.igem.org/Part:BBa_K731721)) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.
+Furthermore, we include T7 promoter  (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.
 <!-- Add more about the biology of this part here