Difference between revisions of "Part:BBa I746907"
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<partinfo>BBa_I746907 short</partinfo> | <partinfo>BBa_I746907 short</partinfo> | ||
| − | This part is used for purification of P7 GFP (for source and other information about this new GFP variant see its part description: I746917) via its 6-his tag. It is driven by a T7 promoter. | + | This part is used for purification of P7 GFP (for source and other information about this new GFP variant see its part description: I746917 and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP) via its 6-his tag. It is driven by a T7 promoter. |
We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged P7 GFP. | We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged P7 GFP. | ||
Latest revision as of 14:57, 28 October 2008
T7 promoter driving 6-his tagged P7 GFP
This part is used for purification of P7 GFP (for source and other information about this new GFP variant see its part description: I746917 and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP) via its 6-his tag. It is driven by a T7 promoter. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged P7 GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 693