Difference between revisions of "Part:BBa I746914"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I746914 short</partinfo>
 
<partinfo>BBa_I746914 short</partinfo>
  
−
This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916) via its 6-his tag. It is driven by the arabinose - sensitive pbad promoter. Induction was with 1mM arabinose for overnight growth of a 50ml culture for small scale purification the next day.
+
This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916 and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP) via its 6-his tag. It is driven by the arabinose - sensitive pbad promoter. Induction was with 1mM arabinose for overnight growth of a 50ml culture for small scale purification the next day.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:55, 28 October 2008

pbad promoter driving 6-his tagged superfolder GFP

This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916 and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP) via its 6-his tag. It is driven by the arabinose - sensitive pbad promoter. Induction was with 1mM arabinose for overnight growth of a 50ml culture for small scale purification the next day.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1249