Difference between revisions of "Part:BBa I746915"

 
 
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<partinfo>BBa_I746915 short</partinfo>
 
<partinfo>BBa_I746915 short</partinfo>
  
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This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916) via its 6-his tag. It is driven by a T7 promoter. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged superfolder GFP.
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This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916 and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP) via its 6-his tag. It is driven by a T7 promoter. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged superfolder GFP.
  
 
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Latest revision as of 14:54, 28 October 2008

T7 promoter driving 6-his tagged superfolder GFP

This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916 and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP) via its 6-his tag. It is driven by a T7 promoter. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged superfolder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 62