Difference between revisions of "Part:BBa K3629018:Design"

 
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===Source===
 
===Source===
  
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The coding sequence of the BGS is from <i>Neocallimastic patriciarum</i>, however it has been codon-optimized and mutated to have optimal activity in <i>Yarrowia lipolytica <i> based on our modelling. The promoter and terminator sequences are from wild-type <i>Y. lipolytica.</i>
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The coding sequence of the BGS is from <i>Neocallimastic patriciarum</i>, however it has been codon-optimized to have optimal activity in <i>Yarrowia lipolytica. </i>The promoter and terminator sequences are from wild-type <i>Y. lipolytica.</i>
  
 
===References===
 
===References===

Latest revision as of 07:33, 18 October 2020


N. patriciarum BGS expression construct with 6X His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3112
    Illegal SpeI site found at 253
    Illegal PstI site found at 218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3112
    Illegal NheI site found at 44
    Illegal SpeI site found at 253
    Illegal PstI site found at 218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3112
    Illegal BglII site found at 660
    Illegal BglII site found at 2641
    Illegal BamHI site found at 973
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3112
    Illegal SpeI site found at 253
    Illegal PstI site found at 218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3112
    Illegal SpeI site found at 253
    Illegal PstI site found at 218
    Illegal NgoMIV site found at 1484
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

x


Source

The coding sequence of the BGS is from Neocallimastic patriciarum, however it has been codon-optimized to have optimal activity in Yarrowia lipolytica. The promoter and terminator sequences are from wild-type Y. lipolytica.

References