Difference between revisions of "Part:BBa I746917"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_I746917 short</partinfo> | <partinfo>BBa_I746917 short</partinfo> | ||
| Line 10: | Line 9: | ||
Its in vivo properties are the same as those of mut3 GFP (for improved in vivo properties see superfolder GFP: I746916). However, it is more stable in vitro and resists denaturation better than mut3. Additionally it refolds (after denaturation) at a much faster rate than does mut3 GFP - hence it might be useful for in vitro applications. | Its in vivo properties are the same as those of mut3 GFP (for improved in vivo properties see superfolder GFP: I746916). However, it is more stable in vitro and resists denaturation better than mut3. Additionally it refolds (after denaturation) at a much faster rate than does mut3 GFP - hence it might be useful for in vitro applications. | ||
| + | |||
| + | More information about the properties of this GFP in relation to the currently used mut3GFP can be found at: http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP. | ||
It has been used in the following constructs: | It has been used in the following constructs: | ||
Latest revision as of 14:51, 28 October 2008
P7 GFP
Coding region of P7 GFP (Fisher et al(2008): "Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control", PLoS ONE 3(6))
P7 GFP carries the following amino acid changes as compared to mut3, the "standard" GFP used in the majority of registry constructs:
F64L, G65A, V68L, N105Y, E124V, Y145F
Its in vivo properties are the same as those of mut3 GFP (for improved in vivo properties see superfolder GFP: I746916). However, it is more stable in vitro and resists denaturation better than mut3. Additionally it refolds (after denaturation) at a much faster rate than does mut3 GFP - hence it might be useful for in vitro applications.
More information about the properties of this GFP in relation to the currently used mut3GFP can be found at: http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP.
It has been used in the following constructs: Driven by pBAD and T7 promoters: I746904 and I746906 respectively. A 6-his tagged version for purification exists and is driven by pBAD or T7 as well: I746905 and I746907 are the respective part numbers.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644