Difference between revisions of "Part:BBa K3629016:Design"
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===Source=== | ===Source=== | ||
| − | The coding sequence of the | + | The coding sequence of the EGI is from <i>Trichoderma reesei</i>, however it has been codon-optimized and mutated to have optimal activity in <i>Yarrowia lipolytica<i> based on our modelling. The promoter and terminator sequences are from wild-type <i>Y. lipolytica.</i> |
===References=== | ===References=== | ||
Revision as of 07:26, 18 October 2020
Modified T. reesei EGI expression construct with gibson homology sequences and 6X His tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2385
Illegal EcoRI site found at 2204 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2204
Illegal NheI site found at 81
Illegal SpeI site found at 2386
Illegal PstI site found at 2400
Illegal NotI site found at 7
Illegal NotI site found at 2393 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2204
Illegal BamHI site found at 2334
Illegal XhoI site found at 132 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2386
Illegal EcoRI site found at 2204 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2204
Illegal XbaI site found at 16
Illegal SpeI site found at 2386
Illegal PstI site found at 2400
Illegal NgoMIV site found at 1326 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
x
Source
The coding sequence of the EGI is from Trichoderma reesei, however it has been codon-optimized and mutated to have optimal activity in Yarrowia lipolytica<i> based on our modelling. The promoter and terminator sequences are from wild-type <i>Y. lipolytica.