Difference between revisions of "Part:BBa K3629013"
Line 3: | Line 3: | ||
<partinfo>BBa_K3629013 short</partinfo> | <partinfo>BBa_K3629013 short</partinfo> | ||
− | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | ===Design=== | |
− | + | ||
+ | ===Sequence and Features=== | ||
<partinfo>BBa_K3629013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3629013 SequenceAndFeatures</partinfo> | ||
Revision as of 07:08, 18 October 2020
Modified PfCBHI expression construct with gibson homology sequences and 6X His tag
Usage and Biology
Design
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2725
Illegal EcoRI site found at 2546 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2546
Illegal NheI site found at 75
Illegal SpeI site found at 2726
Illegal PstI site found at 2740
Illegal NotI site found at 7
Illegal NotI site found at 2733 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2546
Illegal BglII site found at 1322
Illegal BamHI site found at 2631
Illegal XhoI site found at 87 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2726
Illegal EcoRI site found at 2546 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2546
Illegal XbaI site found at 16
Illegal SpeI site found at 2726
Illegal PstI site found at 2740
Illegal NgoMIV site found at 804 - 1000COMPATIBLE WITH RFC[1000]