Difference between revisions of "Part:BBa K3431000:Design"

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===Design Notes===
 
===Design Notes===
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We introduce a silent mutation in order to conform to RFC10 and RFC1000 assembly standards. Specifically, we altered the the sequence of GAATTC into GAATTT to avoid EcoRI restriction sites, and the sequence of GAGAGC into GAGATC to avoid BsaI in the sequence of Beta-Fructosidase.
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We introduce a silent mutation in order to conform to RFC10 and RFC1000 assembly standards. Specifically, we altered the the sequence of GAATTC into GAATTT to avoid EcoRI restriction sites, and the sequence of GAGAGC into GAGATC at 410bp to avoid BsaI in the sequence of Beta-Fructosidase.
  
 
===Source===
 
===Source===

Latest revision as of 03:11, 18 October 2020


Thermotoga maritima Invertase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1078
    Illegal BamHI site found at 1208
    Illegal XhoI site found at 1279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We introduce a silent mutation in order to conform to RFC10 and RFC1000 assembly standards. Specifically, we altered the the sequence of GAATTC into GAATTT to avoid EcoRI restriction sites, and the sequence of GAGAGC into GAGATC at 410bp to avoid BsaI in the sequence of Beta-Fructosidase.

Source

This Beta-Fructosidase came from Thermotoga maritima, a hyperthermophillic bacteria.

References