Difference between revisions of "Part:BBa K103014:Design"
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Preparation of BBa_K103014 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook). | Preparation of BBa_K103014 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook). | ||
| − | [https://parts.igem.org/Part:BBa_K103006 BBa_K103006] was digested with EcoRI and SacI and ligated into [https://parts.igem.org/Part:pSB2K3 pSB2K3] standard BioBrick vector ([https://parts.igem.org/Part:pSB2K3 pSB2K3] with SacI site obtained by EcoRI/SacI digest of [https://parts.igem.org/Part:pSB2K3 pSB2K3] carrying [https://parts.igem.org/Part:BBa_K103017 BBa_K103017] part) | + | [https://parts.igem.org/Part:BBa_K103006 BBa_K103006] was digested with EcoRI and SacI and ligated into [https://parts.igem.org/Part:pSB2K3 pSB2K3] standard BioBrick vector ([https://parts.igem.org/Part:pSB2K3 pSB2K3] with SacI site obtained by EcoRI/SacI digest of standard BioBrick vector [https://parts.igem.org/Part:pSB2K3 pSB2K3] carrying [https://parts.igem.org/Part:BBa_K103017 BBa_K103017] part) |
===Source=== | ===Source=== | ||
Latest revision as of 14:26, 28 October 2008
OmpA_linker_alpha_linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1042
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Preparation of BBa_K103014 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).
BBa_K103006 was digested with EcoRI and SacI and ligated into pSB2K3 standard BioBrick vector (pSB2K3 with SacI site obtained by EcoRI/SacI digest of standard BioBrick vector pSB2K3 carrying BBa_K103017 part)