Difference between revisions of "Part:BBa K077001:Design"
 
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===Design Notes===
 
===Design Notes===
−
Restriction of part [[Part:BBa_R1051|BBa_R1051]] with SpeI and PstI and restriction of [[Part:BBa_C0260|BBa_C0260]] with XbaI and PstI. Those 2 are ligated. So plasmid is the same as the one containig BBa_R1051, pSB1A2.
+
Restriction of part [[Part:BBa_R1051|BBa_R1051]] with SpeI and PstI and restriction of [[Part:BBa_C0260|BBa_C0260]] with XbaI and PstI. Those 2 are ligated. So plasmid is the same as the one containig [[Part:BBa_R1051|BBa_R1051]], pSB1A2.
  
  

Latest revision as of 14:17, 28 October 2008

cI (lambda) promoter with normal RBS and AiiA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 174
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction of part BBa_R1051 with SpeI and PstI and restriction of BBa_C0260 with XbaI and PstI. Those 2 are ligated. So plasmid is the same as the one containig BBa_R1051, pSB1A2.


Source

parts BBa_R1051 and BBa_C0260

References