Difference between revisions of "Part:BBa K3419001:Design"
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===Source=== | ===Source=== | ||
| − | Trichosanthin is from the genome of Trichosanthes kirilowii, also known as the Chinese cucumber. | + | Trichosanthin is from the genome of Trichosanthes kirilowii, also known as the Chinese cucumber. The amino acid sequence was found at: https://www.uniprot.org/uniprot/P09989 |
===References=== | ===References=== | ||
Latest revision as of 19:51, 17 October 2020
Tricosanthin
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 409
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 409
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 409
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 409
Illegal NgoMIV site found at 456 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was originally found in the amino acid sequence form. Thus, that sequence was reverse translated into a DNA sequence, and a stop codon of TGA was added at the end.
Source
Trichosanthin is from the genome of Trichosanthes kirilowii, also known as the Chinese cucumber. The amino acid sequence was found at: https://www.uniprot.org/uniprot/P09989