Difference between revisions of "Part:BBa K103009:Design"

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===Design Notes===
 
===Design Notes===
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[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&arg6=13_October_2008&arg7=14_October_2008&arg8=15_October_2008&arg9=16_October_2008&name=Preparation%20of%20linker_alpha%20%28BBa_K103009%29 Preparation of linker_alpha (BBa_K103009)] (Univeristy of Warsaw 2008 iGEM team notebook)
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Preparation of BBa_K103009 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&arg6=13_October_2008&arg7=14_October_2008&arg8=15_October_2008&arg9=16_October_2008&name=Preparation%20of%20linker_alpha%20%28BBa_K103009%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).
  
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Alpha-linker fragment was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha pACYC177+OmpA-omega-ΔA-alpha] using primers: 5' ATGAATTCGCGGCCGCTTCTAGAGGGAGCTCTG GTGGAAGCGGTGGCGGAGGATC  3' and  5' GCACTAGTATTATTCGCCAGTTAATAGTTTGCG  3'. Resulting fragment was digested with EcoRI and BcuI and ligated into [https://parts.igem.org/Part:pSB2K3 pSB2K3] standard BioBrick vector
 
===Source===
 
===Source===
  

Latest revision as of 14:10, 28 October 2008

linker fused to alpha fragment of TEM beta-lactamase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Preparation of BBa_K103009 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&arg6=13_October_2008&arg7=14_October_2008&arg8=15_October_2008&arg9=16_October_2008&name=Preparation%20of%20linker_alpha%20%28BBa_K103009%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).


Alpha-linker fragment was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha pACYC177+OmpA-omega-ΔA-alpha] using primers: 5' ATGAATTCGCGGCCGCTTCTAGAGGGAGCTCTG GTGGAAGCGGTGGCGGAGGATC 3' and 5' GCACTAGTATTATTCGCCAGTTAATAGTTTGCG 3'. Resulting fragment was digested with EcoRI and BcuI and ligated into pSB2K3 standard BioBrick vector

Source

References