Difference between revisions of "Part:BBa K3425051:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Describe why there is no start codon, link to Type IIS guide
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Since the part is designed to be cloned into the Level 0 backbone pSB1C00 using Type IIS iGEM assembly standard, it lacks ATG start codon. You can read more about how to use this cloning standard in <a href="">Type IIS guide</a> of Uppsala 2020 team.
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Describe why there is stop codon included
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−
 
+
  
 +
This part was synthesized with internal stop codon as a control construct. Functional construct with destabilized
  
 
===Source===
 
===Source===

Revision as of 15:29, 17 October 2020


β-subunit of Qβ replicase with cleavable degradation tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1314
    Illegal XhoI site found at 822
    Illegal XhoI site found at 988
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the part is designed to be cloned into the Level 0 backbone pSB1C00 using Type IIS iGEM assembly standard, it lacks ATG start codon. You can read more about how to use this cloning standard in <a href="">Type IIS guide</a> of Uppsala 2020 team.

This part was synthesized with internal stop codon as a control construct. Functional construct with destabilized

Source

Synthesized.

References