Difference between revisions of "Part:BBa K3394004:Design"

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Artificial sequence - synthetic construct
 
Artificial sequence - synthetic construct
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Paenibacillus sp. ICGEB2008
 
Paenibacillus sp. ICGEB2008
  

Revision as of 14:54, 17 October 2020


Cellulolytic complex [1]: His-Endo5a-GS-CBD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal BamHI site found at 28
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal AgeI site found at 1289
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made another design in which the gene order is different: first the CBD and then the cellulase endo5a.


Source

Artificial sequence - synthetic construct

Paenibacillus sp. ICGEB2008

References

Adlakha, N., Rajagopal, R., Kumar, S., Reddy, V. S., & Yazdani, S. S. (2011). Synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect gut bacterium. Applied and environmental microbiology, 77(14), 4859-4866.

Gupta, S., Adlakha, N., & Yazdani, S. S. (2013). Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli. Protein expression and purification, 88(1), 20-25.