Difference between revisions of "Part:BBa K3610049:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Codon optimization was done with SnapGene
+
Codon optimization done with SnapGene
  
 +
Both mCherry and receptor sequence are codon optimized for C. reinhardtii
  
 +
A HA-tag was fused to the mCherry sequence for Western Blotting.
 +
 +
By having the self-cleaving peptide from the foot and mouth virus added to the sequence, coexpression of different proteins on the same vector is faciliated.
  
 
===Source===
 
===Source===

Revision as of 22:03, 16 October 2020


CORE ectodomain / mCherry N-terminal for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1071
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1917


Design Notes

Codon optimization done with SnapGene

Both mCherry and receptor sequence are codon optimized for C. reinhardtii

A HA-tag was fused to the mCherry sequence for Western Blotting.

By having the self-cleaving peptide from the foot and mouth virus added to the sequence, coexpression of different proteins on the same vector is faciliated.

Source

BBa_K2136017, BBa_K3610012, BBa_K3610011, BBa_K416001, BBa_K1150016, BBa_K3610018

References