Difference between revisions of "Part:BBa K3522004:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
Latest revision as of 15:43, 16 October 2020
SV40 pro-F luc- SV40 polyA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1705
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to meet the assemble standards, we made a synonymous mutation to remove restriction site.
Source
The coding sequence of firefly luciferase comes from the genomic sequence of photinus pyralis.