Difference between revisions of "Part:BBa K3522004:Design"
(→Source) |
|||
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | The coding sequence of firefly luciferase comes from the genomic sequence of photinus pyralis. | |
===References=== | ===References=== | ||
Revision as of 15:42, 16 October 2020
SV40 pro-F luc- SV40 polyA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1705
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to meet the typeIIS standard, we made a synonymous mutation of Glu271Glu to remove SapI restriction site.
Source
The coding sequence of firefly luciferase comes from the genomic sequence of photinus pyralis.