Difference between revisions of "Part:BBa K3408007"
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Used Pnar(BBa_K3408000), RBS(BBa_B0034), phy(ycD)(BBa_E0040) and terminator(BBa_B0015) to express Phy(ycD) under the control of P<sub>nar</sub>. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that Phy(ycD) could be expressed. But when our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> could be suppressed and we couldn’t detect more Phy(ycD). | Used Pnar(BBa_K3408000), RBS(BBa_B0034), phy(ycD)(BBa_E0040) and terminator(BBa_B0015) to express Phy(ycD) under the control of P<sub>nar</sub>. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that Phy(ycD) could be expressed. But when our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> could be suppressed and we couldn’t detect more Phy(ycD). | ||
Revision as of 13:52, 16 October 2020
Pnar-B0034-phy(ycD)-B0015
Used Pnar(BBa_K3408000), RBS(BBa_B0034), phy(ycD)(BBa_E0040) and terminator(BBa_B0015) to express Phy(ycD) under the control of Pnar. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that Phy(ycD) could be expressed. But when our Bacillus subtilis was in an aerobic environment, Pnar could be suppressed and we couldn’t detect more Phy(ycD).
Plasmid profile
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 454
- 1000COMPATIBLE WITH RFC[1000]