Difference between revisions of "Part:BBa K3409007:Design"

 
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===Design Notes===
 
===Design Notes===
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Codon Optimization using the IDT Codon Optimization Software. Codons 547 and 682: GGA (Gly) mutated to GGT (Gly) to remove illegal EcoRI site.  
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Codon Optimization using the IDT Codon Optimization Software. Codons 547 and 682: GGA (Gly) mutated to GGT (Gly) to remove illegal EcoRI site. Codon 140 AGC (Ser) mutated into AGT (Ser) to remove NheI.
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===Source===
 
===Source===

Latest revision as of 13:24, 16 October 2020


mcpB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon Optimization using the IDT Codon Optimization Software. Codons 547 and 682: GGA (Gly) mutated to GGT (Gly) to remove illegal EcoRI site. Codon 140 AGC (Ser) mutated into AGT (Ser) to remove NheI.

Source

Entire gene cluster found on GenBank (KJ563250.1): https://www.ncbi.nlm.nih.gov/nuccore/700369833. Specific sequence for mcpB found on Genbank (AIU94002.1): https://www.ncbi.nlm.nih.gov/protein/700369839. Initially identified in cattle E. coli Strain SSuT-25 plasmid in 2009.

References